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Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research
Article Title: A Mouse Model of Hepatic Ischemia-Reperfusion Injury Demonstrates Potentially Reversible Effects on Hippocampal Neurons and Postoperative Cognitive Function
doi: 10.12659/MSM.912658
Figure Lengend Snippet: Photomicrographs of the immunohistochemical staining for the expression of choline acetyltransferase (ChAT) in the mouse hippocampal CA3 region in the mouse model groups with hepatic ischemia-reperfusion injury and the sham group. ( A ) Light microscopy of the immunohistochemical staining for choline acetyltransferase (ChAT) in tissue sections of the mouse hippocampal CA3 region at 11 days postoperatively. Magnification ×100. ( B ) Light microscopy of the immunohistochemical staining for ChAT in tissue sections of the mouse hippocampal CA3 region at 18 days postoperatively. Magnification ×100. ( a1, a2 ) The sham group, consisting of mice that underwent anesthesia, surgery, and separation of the hepatic artery and vein, but without occlusion. ( b1, b2 ) The I/R1 group, in which the left hepatic artery and portal vein were clamped for 20 min, followed by reperfusion for 30 min. ( c1, c2 ) The I/R2 group, in which the left hepatic artery and portal vein were clamped for 40 min followed by reperfusion for 30 min. Data are presented as the mean ±SD (n=10). ( C ) Comparison between with sham group at day 11 with day 18 after surgery (* P<0.05). Comparison between the I/R1 group at day 11 after surgery with day 18 after surgery ( # P<0.05). Arrows indicate the positively stained cells.
Article Snippet: A rabbit polyclonal antibody to
Techniques: Immunohistochemical staining, Staining, Expressing, Light Microscopy, Comparison